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KMID : 1134820190480010008
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Journal of the Korean Society of Food Science and Nutrition
2019 Volume.48 No. 1 p.8 ~ p.17
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Anti-Obesity Effects of Combined Extracts of Terminalia chebula and Phyllanthus emblica (FBF-TC) in Matured 3T3-L1 Adipocytes and High Fat Diet-Induced Obese Mice
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Park Soo-Jeung
Lee Min-Hee
Yun Jeong-Moon
Kim Da-Kyung
Lim Hyun-Jung
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Abstract
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Abnormal fat accumulation on the body causes chronic disease known as obesity and is a serious problem in today’s society. We investigated the anti-obesity effects of combined extracts of Terminalia chebula (T) and Phylanthus emblica (P) (TP; FBF-TC) in vitro and in vivo. 3T3-L1 preadipocytes were differentiated for 9 days. On the last day of differentiation, Oil red O staining, glycerol release, intracellular cAMP levels, and lipolytic enzymes were measured. Compared to the control, lipid accumulation, and mRNA expressions of PDE3B and Perilipin were significantly decreased in the presence of TP 80 μg/mL. The levels of free glycerol, intracellular cAMP, and mRNA expressions of HSL and ATGL were significantly increased by 139%, 99.5%, 65.6%, and 100.8%, respectively, in the presence of TP 80 μg/mL. In the animal study, C57BL/6J mice were tested for 12 weeks after being separated into eight groups: Normal diet group (ND), high fat diet (HFD) group (C, Control), HFD with 1 g/kg b.w. of catechin group (PC, positive control), HFD with 600 mg/kg b.w. of T or P groups, and HFD with 150, 300, 600 mg/kg b.w. of TP groups. Feeding of HFD for 12 weeks resulted in significant increases in body weight compared to the ND group. FBF-TC groups showed reduced body weight, weight gain, mesenteric white adipose tissue (WAT) weight, subcutaneous WAT weight, serum total cholesterol, LDL-cholesterol, and triglyceride levels compared to the C group. In conclusion, this study suggests that TP is a novel anti-obesity candidate through its stimulation of lipolysis.
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KEYWORD
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Terminalia chebula, Phyllanthus emblica, anti-obesity, 3T3-L1, C57BL/6J
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